Heaven G.

Walking in the halls towards the Fred Hutch training lab is a feeling like no other. It is filled with purpose, foundation, goal, and dedication so strong that even a bystander could sense it. Throughout my time in the Explorers program, I was fortunate enough to be a part of many lab activities. I believe that this part of the program is one of the components that make it unique and full of learning opportunities. For me, a highlight was learning about CRISPR and doing a lab procedure demonstrating this widely known technique. This lab was a demonstration of how scientists and researchers use all basic techniques, such as pipetting and gel electrophoresis, which we learned at the start of the program, to conduct a protocol in modifying DNA. It was new and interesting to have a hands-on experience with this technology that is being used to further aid cancer research.

In simple terms, clustered interspaced short palindromic repeats, or CRISPR, is a molecular scissor that researchers use to “edit” DNA in order to replace and fix genetic mutations that result in diseases such as cancer. In recent years, it has become a force in the biomedical world because of its ability to edit DNA with ease and precision. At Fred Hutch, this tool is being utilized to study genetic mutations and to find better treatments for cancer. Being able to have a first-hand experience on how and why this tool is used has given me a new perspective on how medicine, especially cancer research, is making strides towards better treatments through useful lab applications. In addition, I also learned the value of accuracy and precision on the outcome of research.

Overall, beyond genetic engineering, this experience has made a profound impression on me as it has taught me the passion and dedication it takes to be a researcher and the ways in which careers in this field are evolving beyond just the area of biology. Through guest speakers such as Dr. Barry Stoddard, I learned about the incorporation of both physics and chemistry in cancer research. Many of the lab tours incorporated hands-on experience and helpful information about that particular area of research. Besides, all volunteers shared their background in a way that ignited inspiration among all explorers. Through their unique journeys of getting to their careers, I was able to immerse myself and see my future in science and biology. Furthermore, I learned how researchers tackle mistakes in the lab, as they are unavoidable when it comes to some protocols, some more than others. However, through the lab activities, I learned that observations are key to having a better procedure when preparing for a new protocol. Lastly, I was able to learn about other opportunities at Fred Hutch that I could be a part of in the future. This includes internships, online research programs, and the journal club. Within the two weeks I have been a part of this program, I have done more than I could’ve ever hoped for. One of the outcomes I was hoping for was clarity on my future career choice, and I am glad to say that it has helped me do just that.

In Vitro CRISPR Cas9 gel electrophoresis lab result

An X-ray looking at a crystal protein structure (A lab tour with Dr.Stoddard)

CML Spot Test and Gel Electrophoresis: Testing for Chronic Myeloid Leukemia lab result