Bella Z.

This lab was the dye lab, it represented the separation of molecules based on their charge, shape, and size using the tool of agarose gel electrophoresis. Usually used with DNA and RNA.

This image was not from the same lab but still represents the same concept

First, we make a gel that has pits in it so the tested molecules can sink down at the gel level so it can travel in a controlled yet free & accurate manner. The gel is made with agarose powder boiled with a buffer solution and then later cooled in a mold that forms the pits (or wells). The gel used for showing the molecular difference has tiny pores that dictate how easily the molecules of a substance migrate. This means that smaller molecules move more easily and vice versa for larger ones. The shape also influences the rate of migration. More dense shaped molecules will move through easier while it is vice versa for looser shaped molecules. Lastly, a stronger charge of a molecule will opt for the opposite charge more than a weaker charged molecule. Before the samples are put into the wells, they are mixed with a compact solution (glycerol) so they are more dense than the buffer. As they are more dense, this is what makes them able to sink into the wells. When power is given, the electric pull is activated. Negatively charged molecules make their way to the positive electrode while positively changed molecules go to the negatively charged electrode. The cooled gel is then put inside the middle of the gel box and is filled with buffer solution. The box contains electrodes also which is what forces the difference between the molecules to show. For this lab, the wells on the gel slab. 

Here in the last image, the results show that dye #1 (Bromophenol Blue) is in second place for the most charge/smallest size/most compact & first place for dye #2 (Methylene Blue.) (Disregarding the rest for not being visible, smeared, or too close & similar color to the smeared one to safely tell).

This procedure has brought me to the realization that I enjoy working with the tiny unbelievable processes that build the foundation of science. Part of it also being due to the specific and careful lab procedure process and the working with unfamiliar funky lab equipment with it, it has assured me and completed my program goal of helping me decide if I want to include at least part of it into my career, and that part being that I definitely want to involve the micro & complicated processes of science in it.