Anna D.

This summer, I had the incredible opportunity to be part of the Fred Hutch Pathways Research Explorers Program, where I was immersed in the world of cancer biology and research. Over these two weeks, I engaged in hands-on lab activities, connected with educators and fellow students, and gained insight into real-world research practices. This experience not only deepened my passion for science but also gave me a clearer vision of the many career pathways I can pursue in the future.                  

One of the most memorable experiences I had in the lab was the CML activity.  Chronic Myeloid Leukemia (CML) is caused by a genetic mutation where parts of chromosomes 9 and 22 swap places, creating the ABL, a proto-oncogene, to become fused to the BCR protein. This mutation produces a constantly active tyrosine kinase enzyme that signals white blood cells to grow uncontrollably. The resulting uncontrolled cell division leads to the buildup of white blood cells, replacing healthy cells and causing leukemia. The altered chromosome 22 formed by this translocation is known as the Philadelphia chromosome, a hallmark of CML.                 

In this lab, we were given 4 patient blood samples (which were really just food coloring). Our job was to isolate each patient’s sample DNA and to use gel electrophoresis to detect the BCR-ABL gene, which causes CML. As the bands of each patient’s DNA sample appeared on the gel, we compared it to the bands on the DNA ladder and the control. Therefore, if a specific band appeared, that meant that the presence of the BCR-ABL gene was detected, and they would be diagnosed with CML.

Gel electrophoresis of our CML lab activity

The actual gel of our CML lab activity      

This lab left a lasting impression on me because it transformed a complex cancer like CML into something hands-on. Rather than just reading about the cancer in a textbook, we got to visualize how a small genetic mutation can alter a person’s health so drastically. Watching the DNA bands appear on the gel and interpreting what they meant made the science feel real and immediate. The process of analyzing patient samples, even though they weren’t legit, gave me a sense of responsibility and insight into the steps that real researchers take. It was both fascinating and humbling to realize how precise molecular tools can reveal life-changing information. This activity only deepened my appreciation for cancer biology and strengthened my interest in cancer research. Even after doing this lab at the program, I was so intrigued that I went home to research treatment options for CML. I was fascinated to discover that a treatment called tyrosine kinase inhibitors (TKIs) exists, which specifically blocks the BCR-ABL protein caused by the Philadelphia chromosome. These drugs, like imatinib have transformed CML from a fatal cancer into something manageable for many patients. This revealed to me how research directly translates to hope and healing, and it inspired me to continue exploring this department of science and medicine.

Me and my peers on our first day in the training lab!     

Lastly, I’m incredibly grateful to have been part of this program. This experience has deepened my passion for science and opened my eyes to the world of research in ways I never expected. Thank you to Dr. G, Huda, Alex, Shayan, all the researchers, the science education team, and other high school and undergraduate students who shared their time and knowledge. Your support made these past two weeks both inspiring and unforgettable!